JavaScript is disabled for your browser. Some features of this site may not work without it.
  • About K
  • Academics
  • Admission
  • Alumni Relations
  • Giving to K
  • News & Events
  • Student Life
  • HORNET HIVE
  • ATHLETICS
  • SITEMAP
  • WEBMAIL
    • Login
    View Item 
    •   CACHE Homepage
    • Academic Departments, Programs, and SIPs
    • Biology
    • Biology Senior Individualized Projects
    • View Item
    •   CACHE Homepage
    • Academic Departments, Programs, and SIPs
    • Biology
    • Biology Senior Individualized Projects
    • View Item

    Effects of AS2S2 on DNA Methylation in F-36P Cell Line Derived from Myelodysplastic Syndromes

    Thumbnail
    View/Open
    Searchable PDF/Kalamazoo College Only (430.2Kb)
    Date
    2019
    Author
    Wang, Ruiyao
    Metadata
    Show full item record
    Abstract
    Myelodysplastic syndrome (MDS) is a condition that occurs when the bloodforming cells in bone marrow become abnormal. MDS is considered as a type of cancer that is caused by genetic mutations, abnormal chromosomes, abnormal immune system and DNA methylation. Due to the lack of effective treatments on the market, researchers have inverted to arsenic sulfide (AS2S2), extract from a traditional Chinese formula, Qing Huang powder. In this study, our goal is that the AS2S2 can correct abnormal methylation and promote methylation, aiding in treatment of MDS, which could help MDS patients on their gene expression. We used MDS-derived F-36P cell to test the effect of the AS2S2 on DNA methylation. Furthermore, we used effect of the AS2S2 on DNA methylation of leukemia-derived HL-60 cells to compare with F-36P cells. Our hypothesis is that the AS2S2 has double effects on the DNA, which is regulation of hypermethylation and hypomethylation. The result showed that AS2S2 had double effects, hypermethylation and hypomethylation, which is proved on the DNA of MDS-derived F-36P cell. From 450K chip detection, we received that full-genome wide DNA has methylation. In addition, we tested cell methylation chip detection: full-genome wide DNA methylation in MDSderived Cells F-36P Cells; IC 50 to compare effective between F-36P and HL-60. In the future study, we will test effect on gene methylation on MDS patients, because cell tests could not be able to show the truly effect on patients. Besides, we should also test whether AS2S2 could also help with on the other cancers.
    URI
    http://hdl.handle.net/10920/36745
    Collections
    • Biology Senior Individualized Projects [1550]

    Browse

    All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Login

    DSpace software copyright © 2002-2021  DuraSpace
    DSpace Express is a service operated by 
    Atmire NV
    Logo

    Kalamazoo College
    1200 Academy Street
    Kalamazoo Michigan 49006-3295
    USA
    Info 269-337-7000
    Admission 1-800-253-3602

    About K
    Academics
    Admission
    Alumni Relations
    Giving to K
    News & Events
    Student Life
    Sitemap
    Map & Directions
    Contacts
    Directories
    Nondiscrimination Policy
    Consumer Information
    Official disclaimer
    Search this site


    Academic Calendars
    Apply
    Bookstore
    Crisis Response
    Employment
    Library
    Registrar
    DSpace Express is a service operated by 
    Atmire NV