Effects of AS2S2 on DNA Methylation in F-36P Cell Line Derived from Myelodysplastic Syndromes

Abstract

Myelodysplastic syndrome (MDS) is a condition that occurs when the bloodforming cells in bone marrow become abnormal. MDS is considered as a type of cancer that is caused by genetic mutations, abnormal chromosomes, abnormal immune system and DNA methylation. Due to the lack of effective treatments on the market, researchers have inverted to arsenic sulfide (AS2S2), extract from a traditional Chinese formula, Qing Huang powder. In this study, our goal is that the AS2S2 can correct abnormal methylation and promote methylation, aiding in treatment of MDS, which could help MDS patients on their gene expression. We used MDS-derived F-36P cell to test the effect of the AS2S2 on DNA methylation. Furthermore, we used effect of the AS2S2 on DNA methylation of leukemia-derived HL-60 cells to compare with F-36P cells. Our hypothesis is that the AS2S2 has double effects on the DNA, which is regulation of hypermethylation and hypomethylation. The result showed that AS2S2 had double effects, hypermethylation and hypomethylation, which is proved on the DNA of MDS-derived F-36P cell. From 450K chip detection, we received that full-genome wide DNA has methylation. In addition, we tested cell methylation chip detection: full-genome wide DNA methylation in MDSderived Cells F-36P Cells; IC 50 to compare effective between F-36P and HL-60. In the future study, we will test effect on gene methylation on MDS patients, because cell tests could not be able to show the truly effect on patients. Besides, we should also test whether AS2S2 could also help with on the other cancers.