Development of a.Fluorescence Cleaning Assay for the Detection of Palladium
Kappler, Michael P.
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The purpose of this research was to devise a fluorescence assay suitable to detect palladium in parts per million (ppm) concentrations. The assay had to exhibit consistent linearity and reproducibility so that definitive statements could be made about concentrations of palladium in unknown samples. The main mechanism of the assay is a Tsuji-Trost Oxidative Insertion, in which palladium atoms complex with phosphine ligands in order to bind to and deallylate a chemosensing compound. Before deallylation this compound exhibits low levels of fluorescence when excited by 497 nm wavelength light, while after deallylation it is strongly fluorescent. Good linearity (avg. r2= 0.994), sensititvity (limit of detection= 0.05 ppm palladium in solution) and reproducibility (standard deviation= xxx) were obtained with result. This assay has the potential to be a valuable method in determining preliminary concentrations of palladium in a reactor before any reactions are performed. As this can be done within the company, the overall production process will be faster, cheaper and more convenient than the alternative ICPMS analysis for detection of palladium in a reactor.