Creation of Molecular Tools for the Investigation of Lux Promoter Regulation by a Potential Small RNA Product
Whitney, Joshua J.
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The lux operon within Photorhabdus spp. contains genes sufficient for bioluminescence. The promoter region of this operon, when expressed in the direction upstream of the lux genes, seems to enhance levels of bioluminescence in some systems (Frackman et al., 1990). One explanation for this phenomenon is that the transcription and subsequent translation of the lux promoter in the upstream direction creates a bacterial small RNA (sRNA) product that regulates the activity of the lux promoter in the downstream direction. This project creates molecular tools that could be used to replicate the experiment of Frackman et al. (1990) in order to verify this phenomenon of interest. Another aim of this project is to provide future researchers with molecular tools that could be used to create a dual-plasmid system within the model organism E. coli. This proposed system could be used to investigate the presence of the aforementioned potential sRNA produced by the transcription of the lux promoter in the reverse direction.