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dc.contributor.authorHenning, Jordan
dc.description1 Broadside. Designed using Microsoft PowerPoint. 48"W x 36"Hen_US
dc.description.abstractRibonucleic acid enzymes, or ribozymes, are RNA that is capable of catalyzing biochemical reactions. Hammerhead ribozymes were one of the earliest known classes of ribozymes, and are one of the best characterized. They are of interest for therapeutic use because they can be artificially engineered to bind to and cleave a specific mRNA target sequence with very few off-target effects. Researchers have discovered that minimal hammerhead ribozymes, those without extended stem and loop structures radiating from the core, are still catalytically active. However, cleavage reactions occur with minimal hammerhead ribozymes only at very high, physiologically irrelevant Mg2+ concentrations. It has been hypothesized that if stem I of a minimal hammerhead ribozyme was replaced by the anticodon stem of E. coli tRNA alanine, the cleavage rate of the ribozyme would be significantly increased. The purpose of this study is to determine whether this prediction is correct, and to compare and analyze the dynamics of a wild type hammerhead ribozyme and a hammerhead ribozyme with this mutation.en_US
dc.description.sponsorshipKalamazoo College. Department of Biology. Diebold Symposium, 2015en_US
dc.publisherKalamazoo, Mich. : Kalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Diebold Symposium Presentation Collectionen
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.en
dc.titleThe Effect of a Stem I Substitution on the Cleavage Rate and Efficiency of a Minimal Hammerhead Ribozymeen_US

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  • Diebold Symposium Posters and Schedules [479]
    Poster and oral presentations by senior biology majors that include the results of their Senior Integrated Projects (SIPs) at the Diebold Symposium. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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