RGK Drosophila melanogaster Homologs Effects on Voltage Dependent Calcium Channels in Xenopus laevis Oocytes
Silverman, Louise M.
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The RGK protein family is known for being powerful inhibitors of voltage dependent calcium channels. Drosophila melanogaster (dm) RGK homologs, RGK1 and RGK3S, were subcloned and injected in vitro into Xenopus laevis oocytes. Electrophysiology was performed on oocyte models using a two-microelectrode voltage clamp system in order to measure voltage dependent calcium channel (VDCC) activity. A control group of oocytes was only injected with dm VDCC RNA, and demonstrated average currents over time with the largest average peak current. Average currents of oocytes injected with dm RGK1 RNA remained approximately close to zero as voltage increased and had a significantly lower average peak current than the control group. Oocytes injected with dm RGK3S RNA showed larger average currents over increasing voltage and a larger average peak current than the dm RGK1 RNA group. However, the dm RGK3S group’s average currents over increasing voltage and average peak current were not significantly smaller than that of the control group. Molecules that inhibit voltage dependent calcium channels are potential or current therapeutics for severe cardiovascular and neurological diseases. Therefore, further research into the G-domain and C-domain of dm RGK homologs would provide valuable insight into the structure function mechanisms of RGK proteins and possible therapeutic treatments.