Transport Protein Particle (TRAPP) III Is Implicated in the Regulation of Autophagy
The conserved process of autophagy is a degradation process vital to cellular function. The initial step of autophagy occurs through the formation of the preautophagosomal structure (PAS). Although this step is central to autophagy, its onset and formation remains poorly characterized. The transport protein particle (TRAPP) III subunit Trs85 has been previously implicated with the PAS. This study further characterizes its function. Using confocal microscopy, we employ the use of colocalization studies to identify factors to associate TRAPP and the PAS. Various TRAPP III subunits were viewed to further understand TRAPP III’s role in development of the PAS. Green fluorescent protein-Atg8 was used to indicate the development step of the PAS. TRAPP III subunit Trs85, Trs31, and Bet3 were tested for colocalization to Atg8. Our findings did not identify additional TRAPP subunits involved with autophagy. However, our study supports the association of TRAPP III with the PAS. In addition, TRAPP III is suggested to be involved with the expansion of the PAS into a vesicle.