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dc.contributor.advisorMoore, D. Blaine, 1972-
dc.contributor.advisorMoore, D. Blaine, 1972-
dc.contributor.advisorWang, Jiaqiong
dc.contributor.authorAguilar, Yesenia
dc.descriptionv, 40 p.en_US
dc.description.abstractCervical contusion trauma is the most common form of spinal cord injury (SCI) from which 10% of individuals regain motor function. Pathology of SCI often leads to full quadriplegia or paraplegia due to secondary injury following initial trauma resulting from compression or contusion of the cord. The exploration and use of experimental cervical SCI models are limited. Thus, the current study developed a reproducible rat model of contusive cervical (C5) SCI using the Infinite Horizons (IH) Impactor to induce graded severities. Adult female Fischer rats received C5 laminectomy only (sham group) or laminectomy plus graded contusion injury (150, 200, or 250 kiloDyn (kDyn)). Animals were assessed seven and 14 days post-cervical SCI with a variety of functional tests: IBB forelimb scale, weight-supported hanging and griping strength test, BBB open field locomotion score, gridwalk and catwalk test, and by histological analysis. The pathological change of the injured spinal cord was assessed by Luxol Fast Blue (LFB) - Hemotoxylin and Eosin –(H&E) Staining. Injured animals’ demonstrated loss in gray matter and cell necrosis at the lesion, consistent with severity of SCI. Significant decreases in digit movement, inability to use upper body strength to suspend from a metal rod and for gripping, were all indicative of forelimb dysfunction. Hindlimb dysfunctions were observed through significant decreases in ability of paw placement and coordination, Regularity Index (RI), and stride length. Thus, forelimb and hindlimb locomotor deficits observed in this bilateral cervical SCI model are similar to patients with cervical SCI, suggesting the utility of this model for future investigations of the pathophysiological mechanisms and potential therapeutic approaches for cervical SCI repair.en_US
dc.description.sponsorshipMiller School of Medicine. University of Miami. Miami, Florida.
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Biology Senior Individualized Projects Collection
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
dc.titleFunctional and Histological Outcomes of Bilateral Cervical Spinal Cord Contusion Injury in Fischer Ratsen_US
KCollege.Access.ContactIf you are not a current Kalamazoo College student, faculty, or staff member, email to request access to this thesis.

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  • Biology Senior Individualized Projects [1550]
    This collection includes Senior Individualized Projects (SIP's) completed in the Biology Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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