MicroRNA-101 and its Target Oncogene, Stathmin1, in Metastatic Prostate Cancer
Prostate cancer takes the lives of 25,000 men every year with over 200,000 diagnoses in the United States. With one in six men diagnosed in their lifetime, it is crucial that we look into the etiology of prostate cancer. While there is no single cause of prostate cancer, researchers have observed similarities on a molecular level, namely in gene expression, that may be responsible for uncontrolled cell proliferation among cancers. Specifically, many oncogenes have been linked to prostate cancer through observed changes in expression patterns found in tumors. Stathmin1 is a gene that encodes for the regulatory protein found in microtubules and is thought to have oncogenic activity in metastatic prostate cancer. Overexpression of the protein is found in tumors of patients with aggressive prostate cancer, making it an ideal candidate gene to investigate. MicroRNAs are known to post-transcriptionally regulate gene expression. Expression pattern profiling of microRNA-101 has shown an inverse correlation with Stathmin1 expression. The purpose of this study is to determine the in vitro relationship of microRNA-101 and oncogene Stathmin1 expression. The use of immunoblot analysis, microRNA transfections, luciferase assays, and invasion assays showed that Stathmin1 is responsible for the cell proliferation in prostate cancer and that miRNA-101 regulates this gene to decrease invasion and metastasis. This study provides insight on the microRNA-mediated regulation on oncogenes, which leads to a better understanding of prostate cancer.