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dc.contributor.advisorLangeland, James A., 1964-
dc.contributor.advisorRaymond, Pamela
dc.contributor.authorMcLean, Katherine Zanyk
dc.date.accessioned2013-07-08T17:42:48Z
dc.date.available2013-07-08T17:42:48Z
dc.date.issued2012
dc.identifier.urihttp://hdl.handle.net/10920/28892
dc.descriptionv, 52 p.en_US
dc.description.abstractCone photoreceptors of the retina, which are arranged in a precise planar mosaic array, initiate signals from specific wavelengths of light that travel to the brain and are processed to produce vision. If the retina is damaged or the precise cone array is destroyed, color vision can cease to function, as seen in a wide array of debilitating human diseases such as glaucoma and retinitis pigmentosa. The zebrafish retina was chosen as an in vivo model to study photoreceptor patterning and also to explore the abilities of the zebrafish retina to regenerate after injury. It is currently hypothesized that cell-cell interactions, such as tight junctions between photoreceptors, drive the development of the cone mosaic array. Studying such cell-cell interactions, particularly in real-time, is often hindered by the presence of pigments cells. In this study, a zebrafish lacking pigment in the body and eye was created with the intention of using it as an in vivo retinal model. This zebrafish line, currently called the Triple Mutant line, will be used to create a transgenic zebrafish line expressing a tight junction fusion protein known as GFP-ZO-1, which labels cones with GFP. The cone-specific Rx 3000 UCE promoter derived from Xenopus laevis was identified as the best candidate to drive the tight junction protein in cone photoreceptors. Future work should seek to create the transgenic line, which can then be used for in vivo studies of the role of tight junctions in the patterning and regeneration of the retina. Additionally, widespread use of this Triple Mutant could enable in vivo research on eye and general body systems of the zebrafish.en_US
dc.description.sponsorshipMolecular, Cellular, and Development Biology. University of Michigan. Ann Arbor, Michigan.
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Biology Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Biology;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
dc.titleCreation of a pigment-less zebrafish for in vivo analysis of the role of Zonula Occluden-1 in photoreceptor patterning of the retinaen_US
dc.typeThesisen_US
KCollege.Access.ContactIf you are not a current Kalamazoo College student, faculty, or staff member, email dspace@kzoo.edu to request access to this thesis.


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  • Biology Senior Individualized Projects [1489]
    This collection includes Senior Individualized Projects (SIP's) completed in the Biology Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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