Cross-Species Immunoassay Detection of Immunoglobulin-A in Avian Egg Albumen
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It is known that chicken egg albumen contains Immunoglobulin-A (IgA). By simply using antibodies raised against chicken IgA it was possible to determine whether this antibody would cross-react with IgA in other avian species' egg albumen. Due to a close phylogenetic relationship, alligator egg albumen was analyzed in the same way alongside the avian samples. The same albumen, including alligator, was also analyzed using an antibody raised against chicken serum for a more general cross species detection of proteins. After standardization of protein concentration in all samples, cross reaction of anti-chicken IgA antibody among avian species did occur, with similar banding patterns across all species, which seemed to suggest a fairly conserved IgA structure among birds. Alligator samples did not show bands when analyzed for IgA. Studies using the anti-chicken serum antibody yielded many similarities and differences across species, quantitatively and qualitatively. Alligator samples did show bands similar to avian bands with this antibody suggesting a more conserved protein across species.