In-Vitro Testing of Rituximab in Combination with 2-CdA on Different Lymphoma Infected Cell Lines
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Authors
Liddane, Allison L.
Issue Date
2013
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Non-Hodgkin's Lymphoma (NHL) is the most common form of the lymphomas.
Each form of lymphoma has different biological behaviors and characteristics.
Rituximab (Rtxn) is the drug used most commonly in the treatment of NHL. Rtxn has
been shown to improve patient's survival and delays the developing reoccurrence.
Rituximab has been used alone and in the combination with other drugs, like 2cd-A. In
previous studies, preformed by this lab, rituximab was tested in combination with 2cd-A
in-vivo, and the outcomes were successful. This same combination was then tested invitro
and did not have a positive result. It was assumed that the Rtxn drug would not
work successfully in:-vitro. In this study we tested different lots of rituximab alone and in
combination with 2-CdA oh three different cell lines; and discovered that different lots of
rituximab (solo and in combination) are able to be effective on in-vitro lymphoma cells.
Of the seven Rtxn lots that were tested, in all three cell lines the drugs used in
combination with 2-CdA were more effective than when the drug was used alone. One lot of Rtxn was the most effective over a 96 hour time period when used in combination
in all three cell lines. In future work the drugs and combinations that are effective invitro
will be tested in-vivo in immune deficient mice in order to test if the drug treatment
can withstand an in-vivo environment. Then, hopefully with successful results, there will
be human trials.
Description
iv, 40 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.