Design and Validation of UV-Vis Spectrophotometric and HPLC Methods for Determination of Hydroquinone in 4% Cream Preparations at Elba Laboratories
Minkus, Michael D.
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Ultraviolet-visible spectrophotometric (UV-Vis) and high performance liquid chromatographic (HPLC) methods were designed and validated for quantitative and qualitative determination of concentration for hydroquinone (HQ) cream, with a 4% claimed concentration, produced by Elba Laboratories. HQ is the unique active component in the product. Validation parameters including linearity, accuracy, and precision were used. Concentrated HQ in methanol within the range of 7.14-19.0 µlg/mL could be analytically evaluated by UV-Vis. Skin bleaching creams with concentrations of HQ within the range 0.055-0.120 mg/mL can be accurately analyzed and reproduce results consistently with HPLC. Accuracy was determined by the reproducibility of results through multiple analyses of a single aliquot of cream. Stability and concentration were determined via HPLC analysis. Stability analysis determined that HQ cream degrades in the presence of acid, alkaline, and oxidation catalysts. Ultraviolet (UV) light exposure does not alter the chemical integrity of the cream.