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dc.contributor.advisorKiino, Diane R., 1952-
dc.contributor.advisorShanley, Charles J.
dc.contributor.authorIttiara, Shaun T.
dc.date.accessioned2012-02-17T13:46:21Z
dc.date.available2012-02-17T13:46:21Z
dc.date.issued2002
dc.identifier.urihttp://hdl.handle.net/10920/25060
dc.descriptionv, 26 p.en_US
dc.description.abstractLysyl oxidase is an enzyme produced by vascular smooth muscle cells that regulates collagen and elastin accumulation in a number of tissues. It is postulated that there is an inverse relationship between lysyl oxidase and cell proliferation. It is possible that the phenotype switch between activated fibroblast/SMC secreting matrix and actually proliferating and migrating cells may involve nitric oxide. If this is the case, nitric oxide may be effective in treating endothelial dysfunctions, such as atherosclerosis and arterial restenosis. This study attempts to decipher the effect of nitric oxide on lysyl oxidase mRNA expression. Rat smooth muscle cell cultures were serum-starved for 24 hours prior to treatment with different substances that donated or inhibited nitric oxide. TGF-𝛽1, a cytokine found to up regulate lysyl oxidase in preliminary studies, served as a positive control. Results were analyzed using a novel and exciting technique which allows for the simultaneous analysis of multiple samples by quantitative RT -PCR. Our data suggest that there is no significant correlation between nitric oxide concentration and lysyl oxidase mRNA expression in rat smooth muscle cells in vitro. Nevertheless, in comparison to results from similar studies, this data lends credence to the efficacy of the novel RT-PCR technique.en_US
dc.description.sponsorshipSurgery Research Department. William Beaumont Hospital. Royal Oak, Michigan.
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.relation.ispartofKalamazoo College Health Sciences Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Health Sciences;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder. All rights reserved.
dc.titleThe Effect of Nitric Oxide on Lysyl Oxidase mRNA Expression in Rat SMCs in vitroen_US
dc.typeThesisen_US
KCollege.Access.ContactIf you are not a current Kalamazoo College student, faculty, or staff member, email dspace@kzoo.edu to request access to this thesis.


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