The Effect of Nitric Oxide on Lysyl Oxidase mRNA Expression in Rat SMCs in vitro
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Authors
Ittiara, Shaun T.
Issue Date
2002
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Lysyl oxidase is an enzyme produced by vascular smooth muscle cells that
regulates collagen and elastin accumulation in a number of tissues. It is postulated that
there is an inverse relationship between lysyl oxidase and cell proliferation. It is possible
that the phenotype switch between activated fibroblast/SMC secreting matrix and actually
proliferating and migrating cells may involve nitric oxide. If this is the case, nitric oxide
may be effective in treating endothelial dysfunctions, such as atherosclerosis and arterial
restenosis. This study attempts to decipher the effect of nitric oxide on lysyl oxidase
mRNA expression. Rat smooth muscle cell cultures were serum-starved for 24 hours
prior to treatment with different substances that donated or inhibited nitric oxide. TGF-𝛽1, a cytokine found to up regulate lysyl oxidase in preliminary studies, served as a
positive control. Results were analyzed using a novel and exciting technique which
allows for the simultaneous analysis of multiple samples by quantitative RT -PCR. Our
data suggest that there is no significant correlation between nitric oxide concentration and
lysyl oxidase mRNA expression in rat smooth muscle cells in vitro. Nevertheless, in
comparison to results from similar studies, this data lends credence to the efficacy of the
novel RT-PCR technique.
Description
v, 26 p.
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