Gene Expression Profiles in Prostate Cancer Cells
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The metastasis of prostate cancer, particularly to the bone, can cause significant morbidity. This study was designed to determine expression profiles for seven genes that could potentially impact prostate cancer metastasis. These profiles monitored gene expression in three prostate cancer cell lines, LNCaP, PC3 and DU145, as well as normal cell lines. All three cancer lines colonize human bone implants; PC3 was removed from a metastatic bone lesion, LNCaP from lymph node metastases, and DU145 from brain metastases. Prostate cancer cells were obtained and RNA was extracted. The RNA was then used to make cDNA, which was amplified using designed primers to check for protein expression using RT-PCR. Real-Time PCR will be used to verify these results. The most significant result was the gene expression profile of ADAMI11, a matrix metalloproteinase (MMP). MMPs play roles in both normal bone remodeling and invasion and metastasis of prostate cancer. ADAM11I was greatly expressed in the LNCaP cell line and had almost no expression in the other five lines. This could provide further insight into the metastases of prostate cancer to lymph node and potentially bone. In previous studies by Dr. Michael Cher and colleagues, several MMPs were strongly expressed in PC3 tumor cells, and upon inhibition by batimastat (a broad-spectrum MMP inhibitor), proliferating tumor cells were reduced and degradation of marrow trabeculae within bone was prevented. ADAM11 could provide useful information into the mechanism behind prostate cancer metastasis and can help elucidate the effects of MMPs on cancer growth.