The Role of Cathepsin B in Tumor Progression Involving Collagen I
Martin, Kelly A.
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Cathepsin B (cat B) has been implicated in the degradation of the extracellular matrix. Collagen I is the major component of the bone matrix, which is a component of the extracellular matrix. It has been shown that cathepsin B degrades collagen I. There are also enzymes that degrade the matrix (MMPs and serine pro teases) and they are all members of the proteolytic cascade that plays a role in tumor invasion. The goal of this project was to show the contribution of cathepsin B to tumor invasion. The effects of three different prostate cancer cell lines (DU145, PC3, and LNCaP) on degradation of collagen I were studied. Cancer cells were grown on plastic and three different types of collagen: bovine collagen I, mouse collagen I(+/+), and mutated mousecollagen I (r/r). Expression, secretion and activity of cathepsin B in prostate cancer cells were determined by the means of western blots and fluorescent activity assays were performed to interpret theresults. 0ur data showed that both cathepsin B expression and Ievels of activity increase in this process, which resulted when DU145 cells are grown on bovine or mouse collagen I as compared to plastic. Cathepsin B is capable of degrading both wildtype collagen I (coll +/+), as well as the its mutated form (coll r/r), which is resistant to collagenases (MMPs). This implies that cathepsin B, contributes to tumor progression and metastasis.