Enhanced CCL7 and CCR5 Gene Expression in Usual Interstitial Pneumonia
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Authors
Choi, Esther S.
Issue Date
2004
Type
Thesis
Language
en_US
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Abstract
There has been a struggle to diagnose and manage idiopathic interstitial
pneumonia, where the etiology of the disease is unknown and challenging to investigate.
There has been considerable research effort directed toward elucidating the cellular and
molecular mechanisms through which fibroblasts are triggered and remain activated
during progressive, fibrosing disease of the lung parenchyma. Idiopathic pulmonary
fibrosis (IPF) or otherwise known as Usual Interstitial Pneumonia (UIP) is a relentless
progressive disease, which usually leads to death within 5 years of diagnosis. Symptoms
of UIP diagnosed patients include dyspnea, restrictive lung dysfunction and impaired gas
exchange. It is seen through this study that chemokines are increased and may contribute
to the remodeling events in Idiopathic Interstitial Pneuomonia (lIP). Differentiating
between the different subtypes of lIP is the focus of this study. Therapies for some of the
lIP types have been inefficient and unsatisfactory. Concomitant expression of
inflammatory CC chemokines and their corresponding receptors in surgical lung biopsies
(SLBs) obtained at disease diagnosis was done in order to identify each form of lIP
according to their factors that activate pulmonary fibrosis. Gene Array analysis helped
examine the upper and lower lobe of SLBs from UIP patients. There was an increase in
CCR5 gene expression in the SLBs of the UIP patient group relative to those with nonspecific
interstitial pneumonia (NSIP). Respiratory bronchiolhatory bronchiol ELISA
protein analysis of the SLBs obtained from patients suspected of having a form of lIP
showed increased expression of CCL7 and CCL22 gene in UIP patients and increased
expression of CCL5 (RANTES) in NSIP-fibrotic patients. This study has provided
further support that inflammatory CC chemokine ligands and CC chemokine ligand
receptors are highly expressed in SLBs obtained from lIP patients compared to SLBs
from non-lIP patients.
Description
37 p.
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