Mature Adipocyte-Derived Dedifferentiated Fat Cells Improve Cutaneous Would Healing
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Mature adipocyte-derived dedifferentiatied fat (DFAT) cells are a population of cells that have demonstrated multipotency similar to that of adipocyte derived stem cells (ADSCs) and bone marrow derived mesenchymal stromal cells (MSCs). In this study, we took a preliminary look into DFAT cells' potential to play a role the in wound healing processes in rats compared with that of dermal fibroblasts. On each rat, we performed four excisional wounds and treated one with DFAT cell seed derived from GFP transgenic rats, one with a fibroblast cell seed and left two untreated controls; at day 10 after wounding, tissue samples were taken. Wound area was measured and wounds were stained (HE, Masson-Trichrome) to determine morphological differences between wounds. Immnohistochemistry was performed for DFAT GFP expression and expression of a-smooth muscle actin to show DFAT's differentiation capability. RT-PCR analysis of recovered tissue was also run to measure gene expression of TGF-𝛽, FGF2, VEGF-A, IL-6, collagen-1, fibronectin, and CTGF. It was found that DFAT transplanted wounds were significantly smaller than fibroblast transplanted wounds or controls. DFAT cells were found to remain alive and differentiate into α smooth muscle actin expressing cells in the wounds after transplantation. DFAT also possibly changes gene expression of FGF2, IL-6, collagen-1, fibronectin, and CTGF in wounds as a part of healing. These results can be used as a basis for further research on the wound healing and differentiation properties of DFAT cells.