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dc.contributor.advisorFaulkner, John A.
dc.contributor.advisorKiino, Diane R., 1952-
dc.contributor.advisorBrooks-Herzog, Susan V.
dc.contributor.advisorSalazar, Jay J.
dc.contributor.authorLee, Amber M.
dc.date.accessioned2012-02-14T21:30:55Z
dc.date.available2012-02-14T21:30:55Z
dc.date.issued2009
dc.identifier.urihttp://hdl.handle.net/10920/24992
dc.descriptionvi, 16 p.en_US
dc.description.abstractMuscular atrophy is the result of discontinued use of muscular function for an extended period of time. It causes a reduction in myofiber size, which in turn leads to an overall reduction in whole muscle mass (size). This study involves the use of trichostatin A, a histone deacetylase inhibitor whose primary role is to increase gene transcription by promoting histone hyperacetylation (Minetti, eta/., 2006). Injection of trichostatin A into skeletal muscles increases myofiber size and restoration of muscle performance (Minetti, et a/., 2006). With that known, the effect of trichostatin A on atrophic soleus muscles of healthy mice was studied. Twelve mice were hind limb suspended for 21 days. Upon suspension, 6 of the mice were treated with trichostatin A, while the remaining 6 were untreated and used as a control. Following day 21, the soleus muscles were removed and stored at -80°C. Individually, the muscles were cross-sectioned, stained and analyzed in order to differentiate between the sizes of the fast (type I) and slow (type II) fibers of the treated and untreated muscles. Overall, there was a significant increase in the areas of the muscle fibers that were treated with trichostatin A for 21 days. Trichostatin A can, therefore, be used to effectively increase muscle fiber mass.en_US
dc.description.sponsorshipBiomedical Engineering. University of Michigan. Ann Arbor, Michigan.
dc.description.sponsorshipPhysiology Department. University of Michigan. Ann Arbor, Michigan.
dc.description.sponsorshipMolecular and Integrative Physiology. University of Michigan. Ann Arbor, Michigan.
dc.description.sponsorshipInstitute of Gerontology. University of Michigan. Ann Arbor, Michigan.
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.relation.ispartofKalamazoo College Health Sciences Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Health Sciences;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder. All rights reserved.
dc.titleEffects of Trichostatin A on Mouse Atrophic Soleus Musclesen_US
dc.typeThesisen_US
KCollege.Access.ContactIf you are not a current Kalamazoo College student, faculty, or staff member, email dspace@kzoo.edu to request access to this thesis.


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