Isolation and Characterization of Macrophages from Bovine Bone Marrow
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Authors
Anwar, Mohsen
Issue Date
1992
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
An isolated rabbit antibody was tested in vitro for binding to
man nose receptors on macrophages extracted from bovine bone marrow.
Removal and purification of macrophages from debris and additional cell
types involved collagenase digestion, unit gravity sedimentation,
adherence to glass coverslips, and washing with phosphate buffered
saline. Cells obtained were stained for determination of esterase activity
and immunocytochemically stained (using the primary antibody) by the
avidin-biotin complex (ABC) method. Results suggested that the isolated
rabbit antibody is specific for the macrophage mannose receptor.
Furthermore, cells that stained darkly for both esterase and
immunocytochemical techniques are likely to be macrophages.
viiAn isolated rabbit antibody was tested in vitro for binding to
man nose receptors on macrophages extracted from bovine bone marrow.
Removal and purification of macrophages from debris and additional cell
types involved collagenase digestion, unit gravity sedimentation,
adherence to glass coverslips, and washing with phosphate buffered
saline. Cells obtained were stained for determination of esterase activity
and immunocytochemically stained (using the primary antibody) by the
avidin-biotin complex (ABC) method. Results suggested that the isolated
rabbit antibody is specific for the macrophage mannose receptor.
Furthermore, cells that stained darkly for both esterase and
immunocytochemical techniques are likely to be macrophages.
Description
vii, 29 p.
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