Effects of APO-Lactoferrin on the Minimum Inhibitory Concentrations of Eight Antimicrobials with S. Aureus, S. Uberis, and E. Coli Strains Isolated from Bovine Mammary Glands
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Authors
Dopp, Jason
Issue Date
1992
Type
Thesis
Language
en_US
Keywords
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Abstract
The minimum inhibitory concentrations (MICs) of eight antimicrobials (penicillin,
cloxacillin, cephapirin, ceftiofur, novobiocin, enroflocxacin, erythromycin, and pirlimycin) in
combination with 0, 0.3, and 5.0 mg/ml of apo-Lactoferrin (apo-Lf) were determined against
Staphylococcus aureus, Streptococcus uberis, and Escherichia coli strains isolated from bovine
mammary glands. The MICs of the antimicrobials displayed dramatic increases in MICs from
<0.5 µg/ml in Mueller-Hinton Broth (MHB) to >64.0 µg/ml in MHB supplemented with 5.0
mg/ml of apo-Lf (MHB5) against S. aureus. Similar results were obtained against S. uberis with
the exception of penicillin which demonstrated no increase in the MIC. Finally, the 𝛽-lactams,
enrofloxacin, and pirlimycin displayed no remarkable change in MICs against E. coli, while
erythromycin and novobiocin demonstrated drastic reductions in MICs from 32.0 and > 64.0
µg/ml in MHB to 8.0 and 16.0 µg/ml in MHB5, respectively. Enrofloxacin and pirlimycin
displayed no change in MICs against E. coli. These results indicate that Lf may affect the
activity of antimicrobials used to treat bovine mastitis. This effect may need to be considered
in selecting the most efficacious treatment.
Description
iv, 16 p.
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