Monitoring the Release of Arachidonic Acid in Human Neutrophils During the Process of Degranulation
French, Scott A.
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Human neutrophils, otherwise known as polymorphonucleocytes, are the body's first internal line of defense against foreign molecules. Its processes include identification, phagocytosis, degranulation, and digestion. The process of degranulation signifies a fusion between the phagosome and the specific and azurophil granules of the cell. This fusion allows for the release of superoxide ions which can further digest the phagosome contents. This fusion, however, is mediated by many different factors, most of which are regulated by the G protein. Of these processes, the activation of PLA2 by the G protein is thought to be responsible for the release of arachidonic acid from the membrane, allowing for more fusogenic properties. This study investigated the relationship between the release of arachidonic acid and the release of hydrogen peroxide during the process of degranulation. The findings showed that the release of arachidonic acid is similiar to the hydrogen peroxide release. The findings also showed that when quinocrine is added to the degranulation process, an inhibition of the PLA2 process was seen when added at the 0 time point. These implications are quite important in that it effectively connects the releasing actions of the neutrophil during degranulation with the overall function of the cell.