Development of HPRT-Mutants and the Resultant c DNA by Metabolization of Benzo[a]pyrene-7,8-dihydrodiol
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Authors
Czaiczynski, Brion Jacob
Issue Date
1995
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
The purpose of this study was to investigate the mutagenic
effects of Benzo[a]pyrene-7,8-dihydrodiol (BPD). By using DNA repair
deficient cells from a person diagnosed with xeroderma pigmentosum
(XP), it was then possible to increase the mutagenic effects of a known
toxicant. BPD was added to XP skin fibroblast cells that can actively
metabolize this compound. In order to study the specific effects,
hypoxanthine phosphoribosyltransferase (HPRT) was selected as the
target gene. By variations in media, HPRT- cells were selected. These
cells were used as a template for RT-PCR. Once the HPRT gene cDNA
is produced from these cells, it will then possible to sequence this
mutated gene and find regions of the gene that have a high mutation
frequency. There was success in both creating HPRT- mutants and
making of the subsequent cDNA, however the sequencing portion of
this study has not yet been completed.
Description
iii, 33 p.
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License
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