Role of Antioxidants in the Prevention of Type I Diabetes Mellitus
Stommen, Tracy A-L
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Previous studies have suggested that some antioxidants and blockers of nitric oxide synthase may decrease or delay the incidence of spontaneous Type I diabetes mellitus in animal models and man. U-I08956-A was administered orally (20 mg/kg) with food to 25 diabetes-prone BB/WOR dp rats beginning at 29-30 days of age. An additional 25 diabetes-prone, age-matched BB/WOR dp rats were monitored and given identical diets without U-I08956-A. Serum and urine glucoses were measured once or twice weekly in all animals. The onset of Type I diabetes was determined by the presence of glycosuria, ketonuria, a serum glucose greater than 16.7 m mol/L (300 mg/ dL) and confirmed by pancreatic microscopy. ·Results showed that 19/22 (86.3%) BB/WOR dp rats treated with U-108956-A over a period of ten weeks developed diabetes compared to 14/21 (66.7%) BB/WOR dp rats without treatment (Chi-squared test p = 0.126 and Fisher test p = 0.162). The mean age of onset was 74.2 +/-10 (SD) days of age in the group treated with U-108956-A and 70.7 +/-9.7 (SD) in the control group. Mean glucose levels for control and drug treated groups were 26 and 24.9 m mol/L (468 and 448 mg/dL), respectively. It was concluded that U- 108956-A does not prevent Type I diabetes or delay the onset in the diabetic prone BB /WOR dp rat.Missing p. 29.