Suitability of Human Erythroleukemia K562 Cell Line in an In Vitro Hematological Toxicity Assay
MetadataShow full item record
The purpose of this investigation is to determine whether the K562 cell line is suitable for use in an in vitro hematological toxicity assay. K562 cells were driven to differentiate down an erythroid-like pathway by 2xl0 -7M cytosine B-arabinoside (ara-c) over 96 hours to simulate erythropoiesis. With this control model of erythropoiesis, cells were then treated with a variety of antibiotics in an attempt to assess hematological toxicity. The assay was performed with five antibiotics at the following concentrations: 1, 10, 100, and 1000 uM. Gentamicin was the negative control and chloramphenicol was the positive control, with tetracycline, minocyc1ine, and U-I 00592 as unknowns. Peroxidase staining and growth kinetics measurements of the treatments, as automatically measured by a Cedex machine, were used to assess differentiation. Additionally, flow cytometry was used to measure changes in CD44 expression, apoptosis, and viability of the treatments. The cell line was determined to be inadequate for use in an in vitro hematological toxicity assay, characterized by weak differentiation of the control group, low levels of ara-c-induced peroxidase staining, low changes in CD44 expression, and putative myeloid differentiation ofK562 cells.