The Effects of Arsenite on Mitogen Activated Protein Kinases in Human Keratinocytes
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Authors
Diviney, Erin M.
Issue Date
2000
Type
Thesis
Language
en_US
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Abstract
The effects of arsenite on several Mitogen Activated Protein Kinases (MAPKs) found in Human Keratinocytes (HACAT) were explored. Western Blot analysis was completed using polyclonal antibodies specific to the phosphorylated & panning c-Jun NH2-terminal kinases (JNK)/Stress-Activated protein kinases (SAPK), p38 and Extracellular ligand-Regulated Kinase (MAPK/ERK 1-2). This showed an increase in the phosphorylated amount of the kinase ERK 1-2, but not an overall increase in the total amount of the protein. This signifies an increase in the activity of the MAPK, but not an increase in the production of ERK 1-2. The protein kinase was then shown to be activated by exposure to arsenite. This may signify a pathway by which arsenite may protect the cell from cancer. Although the data was not quantitative, the trends seen warrant further study. This result is consistent with the findings of numerous researchers using different cell lines (Chen et al., 1998; Ludwig et al., 1998; Huang et al., 1999). No increase in either activation or production of the other protein kinases, JNK/SAPK and p38, was observed under these conditions. To show the increase in ERK 1/2, it was necessary to complete several Western Blots and to compare the total amount of protein to the activated amount. From this comparison, a relationship between the exposure to arsenite and the Mitogen Activated Protein Kinases can be established.
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iv, 29 p.
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