Using a Murine Erythroleukemia Cell Line to Evaluate the Potential Toxicity of Test Compounds
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Authors
Knobloch, Hillary N.
Issue Date
2001
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
The potential toxicologic effects of test compounds on hematopoiesis are
examined in preclinical animal studies by evaluating bone marrow and peripheral blood
hematology. If effects on bone marrow are observed, these can be further characterized
using in vitro cell culture systems. Murine erythroleukemia cells (MELC) are a well-characterized
cell line that can be induced to differentiate into erythroid progenitors, one
of the cell lineages present in bone marrow. The effects on MELC differentiation, cell
cycle, and Annexin V (AnnV) and propidium iodide (PI) staining were examined with
test compounds that had demonstrated effects on bone marrow in animals. DRUG 1,
DRUG 2, and chloramphenicol caused dose-dependent increases in double-positive
Ann V and PI MELC, indicating that the cells were in late apoptosis or necrosis. HMBA-induced
MELC were more sensitive to the compound-related increases in Ann V / PI
positive cells. The test compounds did not alter differentiation or cell cycle in HMBA-induced
cells. MELC may be prove to be a useful cell line for the study of test
compounds that demonstrate bone marrow toxicity in preclinical animal studies.
Description
iv, 36 p.
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