The Effects of Antisense Oligodeoxynucleotides against Proteolipid Protein on the Proliferation and Development of Glial Cells
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Authors
Perry, Christine M.
Issue Date
1996
Type
Thesis
Language
en_US
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Abstract
Myelin is an important structure required for the conduction of nerve
impulses in the nervous system. Oligodendrocytes, which are one type of
glial cell in the central nervous system, are the cells that supply the myelin
membrane by extensions of their cytoplasmic processes encapsulating
surrounding axons. Proteolipid protein is an important component required
for the structural integrity of myelin formation. A mutation in this gene has
deleterious effects on myelin, as those seen in the human disease Pelizaeus
Merzbacher. The jimpy mouse, which is devoid of proteolipid protein
production, only accumulates myelin to approximately 5% of the normal
value and shows degeneration of oligodendrocytes. This murine model has
become essential in studying the function of the oligodendrocyte. Antisense
cDNA made against the proteolipid protein strands blocks translation of the
messenger RNA coding for proteolipid protein. This method will reveal the
function of the proteolipid protein in the animal. Jimpy and normal animals
were injected with antisense and evaluated for their effects on
oligodendrocytes. Despite earlier success in cell culture studies, cell counts
reveal that their is no significant change in the quantity of oligodendrocytes
among those animals treated or untreated with antisense strands.
Description
v, 35 p.
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