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dc.contributor.advisorGeary, Timothy G.
dc.contributor.advisorKlein, Ronald D.
dc.contributor.advisorThompson, David P.
dc.contributor.advisorOlexia, Sally L., 1938-
dc.contributor.authorFaremouth, Susanne J.
dc.date.accessioned2012-02-08T20:09:48Z
dc.date.available2012-02-08T20:09:48Z
dc.date.issued1997
dc.identifier.urihttp://hdl.handle.net/10920/24876
dc.descriptionvii, 55 p.en_US
dc.description.abstractThe focus of this project was to pharmacologically and biochemically characterize a CeK (F17c85) gene product through the expression of its cRNA in the Xenopus laevis system. CeK is believed to be a structural homolog to two K+ channel genes, TWIK-1 and TOK-l. The clone was amplified from a Caenorhabditis elegans cDNA library and was transformed into a pCR 2.1, an E. coli vector. The plasmid was then able to be transcribed into cRNA for injection into oocytes from Xenopus laevis. After injection, oocytes were analyzed using 2-microelectrode patch clamping techniques which measure the electrical potential across the cell. The functional expression of CeK was determined using a voltage clamping technique to measure the ionic chemical activity across the oocyte. The insertion of CeK cRNA resulted in little or no expression of a functional K+ channel in the oocyte system.en_US
dc.description.sponsorshipAnimal Health Discovery Unit. Pharmacia and Upjohn Company. Kalamazoo, Michigan.
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.relation.ispartofKalamazoo College Health Sciences Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Health Sciences;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder. All rights reserved.
dc.titleFunctional Reconstitution and Biophysical Characterization of a Putative Caenorhabditis Elegans K+ Channelen_US
dc.typeThesisen_US
KCollege.Access.ContactIf you are not a current Kalamazoo College student, faculty, or staff member, email dspace@kzoo.edu to request access to this thesis.


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