An Examination of the Cell Biological Properties of the Antitumor Agent CC1065 Using Cultured B16 Melanoma Cells
Newell, Kenneth Alan
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The purpose of this project was to examine the effects of CCI065 on cultured B16 melanoma cells. This was done by exposing cells to CCI065 and then examining their abilities to reproduce (survive), grow, synthesize marcomolecules and progress through the cell cycle. Our results show that CCI065 concentrations in the 10 -4 µ/ml range exerted a significant inhibitory effect on all of these processes. CCI065 caused reproductive cell death, inhibited cell growth and inhibited DNA synthesis at similar concentrations. Significantly higher CCI065 concentrations were required to inhibit RNA and protein synthesis. This information suggests that CCI065 may exert its damaging effect(s) on cells by inhibiting the cell's ability to synthesize DNA. It was also seen that CCI065 disrupted the cell progression by slowing the movement of cells through S phase and blocking the movement of cells from G2 + M into G1. Examination of the binding properties of CC1065 revealed that CC1065 binds to the cells very rapidly and irreversibly. CC1065 also appeared to have a high binding affinity for fetal calf serum. In a more general sense, this study verified the extreme potency of CC1065 as an antitumor agent.