Permeabilization of Mouse L1210 Leukemic Cells
Quiñones, Ender J.
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Mouse leukemic L1210 cells were subjected to a cold shock treatment with buffer containing 0.02% Triton X-100 in near isotonic conditions. Subsequently, cells were rendered permeable to exogenously supplied deoxy-ribonucleoside triphosphates to synthesize DNA semiconservatively. DNA synthesis was dependent upon the presence of Mg2+, Na+, all four deoxynucleotides, and ATP. DNA synthesis was not inhibited by 5, 6-dihydro-5-azathymidine or by 1-B-D arabinofuranosylcytosine, but was inhibited by its phosphorylated product, cytosine l-B-D arabinofuranoside 5'-triphosphate.