Anti-Spirochete Antibodies in the Sera of Periodontitis Patients
Duckett, Bruce Devlon
MetadataShow full item record
Five isolates of Treponema (spirochetes) were assayed against healthy patients and periodontitis patients to see if a statement could be made about the relationship between spirochetes, periodontal disease, the humoral immune response, and the antibodies produced using an ELISA-enzyme linked immunosorbant assay with O.5M GuHCI (guanidine hydrochloride) as a mild protein denaturing agent. Periodontitis patients were found to have higher antibody titers than healthy patients. It was also found that sera of periodontitis patients possibly bonded to new antigens in the five isolates of spirochetes (specifically T. socranskii A TCC 35536) and/or produced more antibodies than healthy patients. However, healthy patients produced higher affinity antibodies over periodontitis patients. Thus, it was concluded that healthy patients produced the most effective antibodies; and Treponema (spirochetes) is a bacterium that contributes to the cause of periodontal disease. The new antigens should be studied to better define a relationship between spirochetes, periodontal disease, the immune response, and the antibodies produced.