The Electrophoretic Analysis of Membrane-Associated and Cytoplasmic Proteins in an Escherichia Coli Mutant Lacking L-Leucyl-tRNA:Protein Transferase, its Parent, and Two Revertants
Hosner, Joseph W.
MetadataShow full item record
The protein composition of an Escherichia Colii mutant deficient in L-Ieucyl-tRNA:protein transferase was compared with that of its parent and two revertants. The cells were separated into inner membrane, outer membrane, cytoplasmic, and periplasmic fractions by processes of osmotic shock and differential centrifugation of French Pressed cells. Each fraction was analyzed electrophorectically under denatured conditions in SDS-polyacrylamide gels. The mutant was found to differ from its parent in several inner membrane and outer membrane proteins. The two revertants were found to be not identical and to differ from both the mutant and its parent in certain inner membrane and outer membrane protein bands. The mutant differed slightly from its parent in a few high molecular weight periplasmic proteins. No significant differences were found among the proteins of the cytoplasmic fractions. These results suggest that L-leucyl-tRNA:protein transferase may be involved either directly or indirectly in the synthesis or incorporation of some membrane-associated proteins.
Showing items related by title, author, creator and subject.
Construction of His-tagged ATR fusion proteins: working toward the identification of ATR-interacting proteins Sassack, Mike (Kalamazoo College, 2004)The protein kinase ATR is responsible for initiating signal transmission at cell cycle checkpoints. ATR is a 303 kDa protein containing a carboxy-terminus kinase domain related to PI-3 kinase, and is homologous to the ...
Construction of His-tagged ATR Fusion Proteins: Working toward the Identification of ATR-interacting Proteins Sassack, Mike (Kalamazoo College, 2004)The protein kinase A TR is responsible for initiating signal transmission at cell cycle checkpoints. A TR is a 303 kDa protein containing a carboxy-terminus kinase domain related to PI-3 kinase, and is homologous to the ...
Traumatic Brain Injury Causes Increased Gut Permeability by Attenuation of the Tight Junction Proteins Zonula Occludens Protein-l and Occludin Kroll, Lauren M. (Kalamazoo College, 2009)Millions of individuals sustain traumatic brain injury (TBI) each year in the United States. In many cases of TBI, the body responds with a cascade of progressive illnesses, including systemic inflammatory response ...