A Search for a Subclinical Urinary Protein Marker for Diabetic Nephropathy in Chinese Hamsters and in Type I Human Diabetics Using Two-Dimensional Gel Electrophoresis
Ingebritson, Jean M.
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Diabetes mellitus is a disorder in which the metabolism of J' glucose is impaired. The disease is associated with numerous complications, the most serious of which is diabetic nephropathy. This form of kidney deterioration is characterized by a gradual thickening of the glomerular basement membrane, expansion of the mesangial matrix material, and in the clinically compromised diabetic individual, increased proteinuria. The improper filtration of urinary proteins in Type I diabetics can be induced by moderate exercise. The urinary proteins can be examined qualitatively with two-dimensional gel electrophoresis, and individual variations may be compared. In this study, a search for subclinical urinary markers for diabetic nephropathy in Type I human diabetics who had no obvious clinical signs of kidney disease was made. Diabetic Chinese hamsters were also examined for subclinical markers. A protein marker was found to exist in both humans and Chinese hamsters. The CBUD (Chinese Hamster Urinary Diabetic) protein spot specifically appeared in aged diabetic hamsters. This protein was approximately 42,000 daltons in molecular weight and the spot was dark brown in color. The subclinical marker for diabetic nephropathy in humans was the BDPBDS (Human Urinary Post-Exercise Diabetes Specific) protein, which was nearly 53,000 daltons in molecular weight. Neither of these proteins appeared in nondiabetic controls, and the BDPEDS spot was only observed after exercise.
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