Calcium Dependence in Hepatocellular Injury and Death
Birtman, Mark A.
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The role of the divalent calcium ion in cell injury and death, the subject of much ongoing research, was considered in the present study. The primary hepatocyte culturing system was chosen as the in vitro means of studying the effect of calcium in cell injury and death mediated by a toxin. Two toxins of established mechanisms of toxicity, the ionophore A23187 and lysolecithin, were chosen as the hepatotoxic agents. The results showed that the presence of extracellular calcium enhanced toxic cell death. In an additional set of experiments a calcium binding agent, lodoxamide, was added to determine if the cells could be protected against the effect of calcium. The results from these experiments show a tendency of lodoxamide towards protecting the cells against the effect of calcium.