Studies on the Effect of Glucagon-Like Immunoreactivity (GLI) on Glucose Production in Isolated Rate Hepatic Cells
Farrell, William J.
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GLI, , glucagon-like immunoreactive materials, have been found in elevated levels in both depancreatized and diabetic animals. The biological activity of these materials is still relatively unknown although it is believed that these materials may compete with pancreatic glucagon for binding sites on the membrane of the liver. Isolated liver cells or hepatocytes have proved to be useful tools in the study of the biological actions of various hormones on glucose production in the liver. The isolated cells retain both their structural morphology and their biological activity. In this experiment, hepatic cells were isolated by perfusion of the rat liver with calcium-free Hank's solution containing 0.01% collagenase and 0.08% hyaluronidase in 4% albumin. Pancreatic glucagon 3485 and GLI materials, molecular weights 9,000 and 12,000 were added to the cell suspensions to study the effect of these hormones on glucose production in the isolated hepatocytes. In cell preparations in which glucagon· was added, there was an overall increase in glucose production by the hepatocytes as compared to a control in which no hormone was present. Although statistical analysis of the data showed no significant difference between the control and the GLI materials, the data did seem to indicate a trend in that these GLI materials may inhibit glucose production in the isolated cells.