Show simple item record

dc.contributor.advisorRoop, Dennis R.
dc.contributor.advisorNewton, Carolyn R.
dc.contributor.authorFlaharty, Patrick M.
dc.date.accessioned2012-02-03T15:17:42Z
dc.date.available2012-02-03T15:17:42Z
dc.date.issued1982
dc.identifier.urihttp://hdl.handle.net/10920/24790
dc.descriptioniv, 29 p.en_US
dc.description.abstractComplementary DNA (cDNA) was synthesized to total poly(A) RNA (mRNA) isolated from newborn mouse epidermis and used to construct recombinant plasmids. Recombinants containing cDNA inserts corresponding to the 67 Kd, 59 Kd, and 55 Kd keratins, which are the major keratins synthesized in this tissue have been identified. By RNA blot analysis from newborn mouse epidermis, RNA species have been identified that are approximately 2400, 2000, and 1800 nucleotides in length and are complementary to the 67 Kd, 59 Kd, and 55 Kd cDNA's respectively. Using these cDNA's as probes, this project entails the isolation and characterization of the natural keratin genes corresponding to the 67 Kd, 59 Kd, and 55 Kd keratin· proteins. The source of the natural genes was a mouse gene library recombinantly constructed in bacteriophage vector Charon 28.en_US
dc.description.sponsorshipNational Cancer Institute. Bethesda, Maryland.
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.relation.ispartofKalamazoo College Health Sciences Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Health Sciences;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder. All rights reserved.
dc.titleIsolation of Natural Keratin Genes from a Mouse Gene Libraryen_US
dc.typeThesisen_US
KCollege.Access.ContactIf you are not a current Kalamazoo College student, faculty, or staff member, email dspace@kzoo.edu to request access to this thesis.


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record