Isolation of Natural Keratin Genes from a Mouse Gene Library
Flaharty, Patrick M.
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Complementary DNA (cDNA) was synthesized to total poly(A) RNA (mRNA) isolated from newborn mouse epidermis and used to construct recombinant plasmids. Recombinants containing cDNA inserts corresponding to the 67 Kd, 59 Kd, and 55 Kd keratins, which are the major keratins synthesized in this tissue have been identified. By RNA blot analysis from newborn mouse epidermis, RNA species have been identified that are approximately 2400, 2000, and 1800 nucleotides in length and are complementary to the 67 Kd, 59 Kd, and 55 Kd cDNA's respectively. Using these cDNA's as probes, this project entails the isolation and characterization of the natural keratin genes corresponding to the 67 Kd, 59 Kd, and 55 Kd keratin· proteins. The source of the natural genes was a mouse gene library recombinantly constructed in bacteriophage vector Charon 28.