Methods for Detecting Abnormalities in Chemotaxis
Beemer, W. Howard
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Through chemotaxis, leukocytes are recruited to inflammatory sites, thereby constituting a central role in the host-defense system. Recent reviews emphasize the association between abnormalities in chemotaxis and an increased incidence and/or severity of infectious disease and neoplastic disease. The correlations drawn between defective chemotaxis and an impressive number of clinical conditions are of little importance if they cannot eventually be translated into practical clinical application. To this end we have developed methodology for the study of two types of humoral chemotactic abnormalities: defects in the generation of serum chemotaxins and the presence of high levels of chemotactic factor inactivators. Using a modified version of the agarose plate technique, it was determined that inadequate chemotaxin generation could be best detected by measuring leukocyte migration elicited by serum treated with zymosan. Levels of chemotactic factor inactivators were best evaluated by quantifying leukocyte migration in response to MET-LEU-PHE-treated serum as presented in a dose-response curve.