Effects of Herbicide U-27267 on Hepatic Glutathione Content in B6C3Fl Mice
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The Upjohn herbicide U-27267 (3,4,5-Tribromo-N-N-a-trimethyl-pyrazole-l-acetamide) was administered once orally via gastric intubation to three groups of B6C3Fl (CXH) mice at doses of 250, 500, and 1000 mg/kg body wt (10 males/group) in a sterile suspension. A negative control consisting of sterile suspension (Sterile Vehicle #122, 0.025% Carboxymethylcellulose) was intubated to one group of B6C3Fl (CXH) mice (10 males). A positive control of 375 mg acetamfnopfen/kg body wt [N-(4-Hydroxyphenyl)-acetamide] was fntubated, also, to one group (10 male CXH mice). All mice were sacrificed at one hour post-treatment, their livers homogenized, and the resulting final nonprotein sulfhydryl contents (NPSH), primarily glutathiones (GSH or a-glutamylcysteinglycine), were measured spectrophotometrically. The results indicated insignificant difference between the U-27267 dose levels and negative control or between the dose levels themselves. All five groups varied substantially from the acetaminophen treatment which at this dose level had been shown to reduce GSH content significantly. Comparing these results to those of an earlier chronic toxicity study of U-27267 wherein doses of 100 and 300 mg/kg body were found to cause liver tumors in ICR mice over a twenty-two month period showed no positive correlation between liver GSH content depression and tumor formation for U-27267.