Three Assay Methods in the Detection and Study of Murine Tumor Immunity
Hawke, Jeffrey E.
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The nature of immunological resistance to tumor grafts in experimental animals can best be studied by harnessing the particular phenomenon in an experimental system. In this paper, three methods of detecting various aspects of immunological reactions against tunor transplants in mice are compared. The tumor used in this study was chosen for a definite reason; it has been shown that growth of explants of the Cloudman (S-91) melanoma are inhibited by pretreating host mice with vitamin A prior to tumor graf'ting. By characterizing the mode of resistance that immunized mice express against an S-91 tumor challenge, new lines of investigation into possible enhancing effects of vitamin A can be pursued. At the same time, comparing and contrasting the results of three experimental methods (the Winn assay, cytotoxicity assay and migration inhibitory factor (MIF) assay) reveal some advantages to using one procedure over another in this particular system.