9-Hydroxy-Prostaglandin-Oxidoreductase Activity in the High Speed Supernatant of Adult Rat Kidney Homogenates
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Studies were performed in order to determine the enzymatic step at which interconversion of PGF to PGE takes place. Radioactive PGF2α substrate was incubated with the dialyzed high-speed supernatant of adult rat kidney homogenates. It was determined that PGF2α could be directly converted into PGE2. The enzyme responsible for this conversion, 9-hydroxy-PG- oxidoreductase, had little cofactor specificity with respect to NAD and NADP.