Studies on the Internal and Extyernal Asparaginases of Saccharomyces Cerevistae
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Authors
Ban, David J.
Issue Date
1976
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Studies were undertaken to further characterize
the constitutive internal form of asparaginase in Saccharomyces
cerevisiae in terms of its genetics and biochemistry and to
show that a precursor-product relationship did not exist .
between this enzyme and the inducible external form found in
three S. cerevisiae strains. A recombinant strain was
isolated that exhibited normal external enzyme activity, but
no internal enzyme activity. A rough estimate of the
molecular weight from two enzyme preparations was obtained,
as were data concerning temperature dependence and sensitivity,
susceptibility to inhibitors and substrate specificity.
Description
iv, 54 p.
Citation
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License
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