Studies on the Internal and Extyernal Asparaginases of Saccharomyces Cerevistae
Studies were undertaken to further characterize the constitutive internal form of asparaginase in Saccharomyces cerevisiae in terms of its genetics and biochemistry and to show that a precursor-product relationship did not exist . between this enzyme and the inducible external form found in three S. cerevisiae strains. A recombinant strain was isolated that exhibited normal external enzyme activity, but no internal enzyme activity. A rough estimate of the molecular weight from two enzyme preparations was obtained, as were data concerning temperature dependence and sensitivity, susceptibility to inhibitors and substrate specificity.