Studies on the Internal and Extyernal Asparaginases of Saccharomyces Cerevistae
Ban, David J.
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Studies were undertaken to further characterize the constitutive internal form of asparaginase in Saccharomyces cerevisiae in terms of its genetics and biochemistry and to show that a precursor-product relationship did not exist . between this enzyme and the inducible external form found in three S. cerevisiae strains. A recombinant strain was isolated that exhibited normal external enzyme activity, but no internal enzyme activity. A rough estimate of the molecular weight from two enzyme preparations was obtained, as were data concerning temperature dependence and sensitivity, susceptibility to inhibitors and substrate specificity.