Characterization of a Transformed Mouse Cerebral Microvessel Endothelial Cell Line for In Vitro Study of Angiogenesis
Hickman, Kathryn Anne
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Angiogenesis is the formation of new capillary blood vessels. This is an important action in many systems, but particularly in the formation of solid tumors. Because tumors will not grow without these capillaries, the study of vascularization is very important. The study of this vascularization can be performed in vivo and in vitro. Cultured endothelial cells, specifically those from microvessels, serve as a good in vitro model to study the process of angiogenesis. This study was developed to characterize a culture of transformed mouse cerebral microvessel endothelial (TEn) cells according to normal endothelial cell markers and in response to growth factors, growth regulators, and growth inhibitors. Results showed that these TEn cells did not require outside factors for growth and had lost certain endothelial cell markers. Therefore, it is still unknown whether these TEn cells will serve as a good model for the study of angiogenesis.