Efficiency of Various Sampling and Dispersion Techniques in the Recovery of Dental Plaque and Pure Cultures of Oral Bacteria
Pure cultures and human dental plaque samples of various oral bacteria were subjected to a range of dispersal techniques: vortexing, sonication and tekmar c. Observations were made as to the percent recovery of viable bacteria from each of the mechanical devices. Special emphasis was placed on the effect each technique had. on the subgingival gram negative organisms, e.g. Fusobacteria Nucleatum and Trepinema Denticola. The method of sampling patient plaque was also investigated. Endodontic paperpoints were contrasted with the standard curette· method for percent recovery of viable bacteria and total bacteria. Results showed the following order of priority in the percent recovery of total viable oral bacteria and F. Nucleatum: tekmar, sonication, and vortexing. This ranking is based on the relationship between the number of colony forming units on an agar medium divided by the number of bacteria observed, prior to dispersion, with a darkfield microscope. The endodontic paperpoint was observed to recover a higher percentage of viable bacteria from patient plaque, but a lower total bacteria count when compared to the standard curette technique.