The Development of a Protocol for Determining the Effects of Vascularization in the CNS on Myelination
Hieshetter, Janet L.
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The presence of myelin is essential in the Central Nervous System. The absence of myelin causes uncoordination of movement and eventually death. The exact factors influencing myelin formation are not known. One factor that may affect myelination is the vascularization of nervous tissue. In this study, the blood-brain barriers of normal, myelin producing mice were compared to Jimpy, a non-myelinated strain of mice. A procedure using horseradish peroxidase (HRP) as a tracer and 3,3-diaminobenzidine (DAB) as the oxidizing reagent in locating the site of the blood-brain barrier was developed for our project. The results indicated a possible break-down or immaturity in the Jimpy mice when compared to the normal mice. Such a breakdown or malfunction of the blood-brain barrier might allow for the permeation of substances into the nervous tissues. These substances normally prevented from entering the nervous tissue, may possibly be of such significance as to affect the formation of myelin.