The Role of Leukocytes in Venous Thrombosis
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Authors
Koets, Mark H.
Issue Date
1982
Type
Thesis
Language
en_US
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Abstract
Deep vein thrombosis is a complication and coexisting
condition of many pathological situations and illnesses.
Its sequel, pulmonary embolism, can develop into a life
endangering situation. At the present time, there is no
ideal diagnostic test or satisfactory treatment of this
condition. The formulation and use of an animal model would
increase our understanding of deep vein thrombosis and aid
in the development of preventative measures.
A model of DVT was developed. Nine cats used in the study
were divided into experimental and control groups. All cats
were anesthesized and local trauma was produced through
exposure of the jugular vein. In the experimental group,
the jugular vein was occluded for 72 hours before removal.
In the control group, the vessel .as removed immediately
after resection of adherent fat and connective tissue. The
vein segments were examined for adherent cells, fibrin, and
thrombi.. Leukocytes were the most abundant. cell type and
appeared to be the first of the blood cell types to arrive.
Leukocytes were often found in association with platelets,
damaged endothelium, and thromhi. Platelets were adherent
to exposed subendothelium and were also found in the fibrous
network of thrombi.
The etiology of DVT is believed to involve hypercoaqulation,
a vascular factor, and venous stasis. Analysis of these
three factors as they pertain to this model provided a
proposed sequential understanding of thrombus formation.
Experimental evidence suggests that adherent leukocytes are
active in thrombus formation. Thrombus formation may be
initiated through endothelial damage, secretion of
leukotrienes, generation of procoagulant activity (PCA),
production of thrombin-like ma t.e rial. (TLB), or through
release of platelet activating factor (PAF).
Stasis and tissue injury produced a reproducible model which
may provide insight into the many cases of spontaneous DVT.
Description
vi, 45 p.
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