A Study of Immune Cell Supernatant-Induced Transient Characteristics of MTF7 and MTF7(T11).2 Mammary Adenocarcinoma Cells
Howrey, Richard P.
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13762NF mammary adenocarcinoma cell clone MTF7 and MTF7(Tl1).2 were grown in various media ( 10%, and 0.125% Fetal Bovine Serum (FBS), Macrophage-Conditioned Media (MCM), and Neurtophil-Conditioned Media (PCM)) for 48 hours prior to interveinous injection into synergeic rats. The rats were then sacrificed 23 days later, the lungs were removed and the metastases were counted and measured. No significant difference was observed between the various treatments. The actin arrangement in the cells, and the in vitro doubling time were also unchanged. The tumor cell morphology, however, was changed. In addition, the in vitro saturation density and the ftbronectin staining patterns were also altered by various media. The MCM and the PCM both caused the cells to become cytostatic.