The Introduction of a Transposable Element, Tn916, into Streptomyces via Recombinant DNA
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The transposable element Tn918 was introduced into the bacterial genus streptomyces so as to produce tetracycline resistant cells. The transposon was excised from the plasmid pAM12B and inserted onto the plasmid pKT819. pKT619 served as the cloning vector. The Tn916-pKT619 chimera was used to transform two strains of streptomyces, S. lividans and UC3~631. These strains unfortunately did not exhibit resistance to the antibiotic tetracycline, as desired (marker for successful t2ansformation). This experiment was of importance because transposable elements have not been observed in Streptomyces, a bacterium which produces over eighty-five percent of the marketed antibiotics. The ability to transform this bacterium may produce long range results including the synthesis of new antibiotics.